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Prime Pro 48 qPCR Machine / PRIMEPRO48

• 40 cycles in 40 minutes before optimisation
• 40 cycles in 15 minutes when optimisation
• HRM - Identify Class IV SNP >99.9% of the time
• Set up a 4 plex reaction in only 4 mouse clicks

• MIQE compliant
• World leading uniformity ±0.1°C across the block
• 400 analyte specific qPCR reagent kits available
• Demo equipment available to evaluate in your own lab

• Consistent results provide trust and confidence
• Compile data from multiple experiments. Pool plates and results across labs
• Record every well, with every filter, in every cycle. Never miss a data point
• Adaptive LED Control means you never saturate the detector, never get crosstalk and and maximise the wide dynamic range
• If you quantify libraries for Next Generation Sequencing with any other block based qPCR system than Prime Pro 48, you risk significant cost implications

• No other qPCR system offers so much performance for such a low price
• Available as a complete system - instrument, plates, seals, reagents
• Chemically open platform - use Techne or any other qPCR reagents
• Lowest price per run - use duplicates and 5-20ul sample volumes. Don't waste money
• Everything as standard including HRM and unlimited software licence 
• Highly uniform qPCR means successful quantification for NGS

• ±0.1°C uniformity across the whole block instantly after every temperature change
• HRM - Identify Class IV SNP >99.9% of the time
• Unsurpassed block uniformity

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Prime Pro 48 qPCR Machine

Full product specification.move

Full product specification.

Volume per well Validated for 5 to 20 ul
Detection sensitivity 1 copy
Temp uniformity +- 0.1 degC within 1 sec
Temp stability +- 0.1 degC
Temperature range 30 to 100 degC
Average ramp rate 5.5 degC sec
Thermal system Hollow silver block peltier-based with conductive fluid
Block format 48-well block
Consumables 48-well custom plates and optical adhesive seals
Optical system Dual LED excitation (452 to 486 nm and 542 to 582 nm)
Camera CCD camera
Filters 4 emission filters (505 to 545nm 562 to 596nm 604 to 644nm 665 to 705nm)
Calibrated dyes SYBR FAM HEX ROX Cy5
Additional dyes All dyes within range compatible with no additional calibration
Passive reference dyes Use of ROX is supported but optional
Data collection Data always collected in all four filters for all wells
Plate setup Plate setup for data analysis can be altered after run completes
Melt curve Supports continuous data acquisition in a single filter to provide increased data point collection and reduced run times
PCR cycle time (unoptimised) 40 cycles in less than 40 minutes
PCR cycle time (FAST optimised) 40 cycles in less than 20 minutes
Dynamic range Greater than 9 logs
Calibration Not required
Installation Plug and play design
Precision Discriminates 5000 and 10000 template copies with 99 percent confidence
Warranty 1 year (parts and labour included)
Voltage 100 to 240 V.AC
Frequency 50 and 60 Hz
Nominal current draw 5A